Boundaries of somatic mutation in rearranged immunoglobulin genes: 5' boundary is near the promoter, and 3' boundary is approximately 1 kb from V(D)J gene.

Abstract excerpt

To investigate why somatic mutations are spatially restricted to a region around the rearranged V(D)J immunoglobulin gene, we compared the distribution of mutations flanking murine V gene segments that had rearranged next to either proximal or distal J gene segments. 124 nucleotide substitutions, nine deletions, and two insertions were identified in 32,481 bp of DNA flanking the coding regions from 17 heavy and...

Topics

• Animals
• Base Sequence
• Gene Rearrangement
• Genes, Immunoglobulin
• Immunoglobulin Joining Region
• Immunoglobulin Variable Region
• Mice
• Mice, Inbred BALB C
• Molecular Sequence Data
• Multigene Family