Article
Distribution of mutations around rearranged heavy-chain antibody variable-region genes.
Molecular and cellular biology - 1 Oct 1990
Both G W, Taylor L, Pollard J W, Steele E J
Abstract excerpt
The mechanism of somatic hypermutation in the variable region of immunoglobulin genes expressed in mammalian B cells is a major unexplained phenomenon in the generation of diversity in the immune system. To evaluate possible mechanisms, the distribution of somatic mutations was examined for a gro...
Topics
- Animals
- Antibody Diversity
- Base Sequence
- Cloning, Molecular
- Gene Rearrangement, B-Lymphocyte, Heavy Chain
- Genes, Immunoglobulin
- Hybridomas
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
- Mice
- Mice, Inbred C57BL
- Molecular Sequence Data
- Mutation
- Oligonucleotides
- Polymerase Chain Reaction
- RNA, Messenger
- Regulatory Sequences, Nucleic Acid
- Transcription, Genetic