Article
Alanine screening mutagenesis establishes the critical inactivating damage of irradiated E. coli lactose repressor.
Radiation research - 1 Jun 2012
Goffinont Stephane, Villette Sandrine, Spotheim-Maurizot Melanie
Abstract excerpt
The function of the E. coli lactose operon requires the binding of lactose repressor to operator DNA. We have previously shown that γ rradiation destabilizes the repressor-operator complex because the repressor loses its DNA-binding ability. It was suggested that the observed oxidation of the four tyrosines (Y7, Y12, Y17, Y47) and the concomitant structural changes of the irradiated DNA-binding domains...
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