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Article

Structure of a variant of lac repressor with increased thermostability and decreased affinity for operator.

Journal of molecular biology - 12 Oct 2001

Bell C E, Barry J, Matthews K S, Lewis M

Abstract excerpt

A single amino acid substitution, K84L, in the Escherichia coli lac repressor produces a protein that has substantially increased stability compared to wild-type. However, despite the increased stability, this altered tetrameric repressor has a tenfold reduced affinity for operator and greatly decreased rate-constants of inducer binding as well as a reduced phenotypic response to inducer in vivo. To understand...

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Source

Identifiers

PMID: 11601849
DOI: 10.1006/jmbi.2001.5041

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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