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Article

Altered specificity in DNA binding by the lac repressor: a mutant lac headpiece that mimics the gal repressor.

Chembiochem : a European journal of chemical biology - 1 Sept 2005

Kopke Salinas Roberto, Folkers Gert E, Bonvin Alexandre M J J, Das Devashish, Boelens Rolf, Kaptein Robert

Abstract excerpt

Recognition of the lac operator by the lac repressor involves specific interactions between residues in the repressor's recognition helix and bases in the DNA major groove. Tyr17 and Gln18, at positions 1 and 2 in the lac repressor recognition helix, can be exchanged for other amino acids to generate mutant repressors that display altered specificity. We have solved the solution structure of a protein-DNA complex...

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Identifiers

PMID: 16094693
DOI: 10.1002/cbic.200500049

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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