Article
Evidence for an essential catalytic role of the F10 protein kinase in vaccinia virus morphogenesis.
Journal of virology - 1 Jan 2004
Szajner Patricia, Weisberg Andrea S, Moss Bernard
Abstract excerpt
Temperature-sensitive mutants of vaccinia virus, with genetic changes that map to the open reading frame encoding the F10 protein kinase, exhibit a defect at an early stage of viral morphogenesis. To further study the role of the enzyme, we constructed recombinant vaccinia virus vF10V5i, which expresses inducible V5 epitope-tagged F10 and is dependent on a chemical inducer for plaque formation and replication. In...
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