Article
A naturally thermolabile activity compromises genetic analysis of telomere function in Saccharomyces cerevisiae.
Genetics - 1 May 2012
Paschini Margherita, Toro Tasha B, Lubin Johnathan W, Braunstein-Ballew Bari, Morris Danna K, Lundblad Victoria
Abstract excerpt
The core assumption driving the use of conditional loss-of-function reagents such as temperature-sensitive mutations is that the resulting phenotype(s) are solely due to depletion of the mutant protein under nonpermissive conditions. However, prior published data, combined with observations presented here, challenge the generality of this assumption at least for telomere biology: for both wild-type yeast and...
Topics
- Alleles
- Microbial Viability
- Mutagenesis
- Mutation
- Phenotype
- Protein Structure, Tertiary
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Telomere
- Telomere Shortening
- Temperature