Article
PNPLA3 is regulated by glucose in human hepatocytes, and its I148M mutant slows down triglyceride hydrolysis.
American journal of physiology. Endocrinology and metabolism - 15 May 2012
Perttilä Julia, Huaman-Samanez Carolina, Caron Sandrine, Tanhuanpää Kimmo, Staels Bart, Yki-Järvinen Hannele, Olkkonen Vesa M
Abstract excerpt
Liver fat is increased in carriers of the minor G allele in rs738409 (I148M amino acid substitution) in patatin-like phospholipase domain-containing 3 (PNPLA3)/adiponutrin. We studied transcriptional regulation of PNPLA3 in immortalized human hepatocytes (IHH) and human hepatoma cells (HuH7) and the impact of PNPLA3 I148M mutant on hepatocyte triglyceride metabolism. Studies in IHH showed that silencing of the...
Topics
- Amino Acid Substitution
- Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
- Cell Line
- Glucose
- Hepatocytes
- Humans
- Lipase
- Lipogenesis
- Membrane Proteins
- Mutation
- Statistics, Nonparametric