Article
Mammalian drug resistant mutants with multiple gene amplifications: genes encoding the M1 component of ribonucleotide reductase, the M2 component of ribonucleotide reductase, ornithine decarboxylase, p5-8, the H-subunit of ferritin and the L-subunit of ferritin.
Biochimica et biophysica acta - 23 Oct 1990
Hurta R A, Wright J A
Abstract excerpt
Hydroxyurea was used to select two very highly drug resistant cell lines, designated HR-15 and HR-30. Both drug resistant lines contained elevated levels of ribonucleotide reductase activity. Northern and Southern blot analysis indicated that the two drug resistant lines contained increased level...
Topics
- Animals
- Blotting, Northern
- Blotting, Southern
- Blotting, Western
- Cell Line
- Drug Resistance
- Ferritins
- Gene Amplification
- Gene Expression
- Hydroxyurea
- Mutation
- Ornithine Decarboxylase
- RNA, Messenger
- Ribonucleotide Reductases