Article
Quantification of ribonucleotide reductase expression in wild-type and hydroxyurea-resistant cell lines employing in situ reverse transcriptase polymerase chain reaction and a computerized image analysis system.
Analytical biochemistry - 1 Feb 1999
Wadler S, Zhang H, Cammer M, Hu X P
Abstract excerpt
Ribonucleotide reductase (RR) is the enzyme responsible for converting nucleoside diphosphates to deoxynucleoside diphosphates, ensuring a balanced supply of deoxyribonucleotides for DNA synthesis. Expression of RR is tightly regulated, but it is affected by exogenous agents, such as hydroxyurea...
Topics
- Base Sequence
- Cell Line
- DNA
- DNA Primers
- Drug Resistance
- Gene Expression Regulation, Enzymologic
- Genetic Variation
- Humans
- Hydroxyurea
- Image Processing, Computer-Assisted
- RNA, Messenger
- Reverse Transcriptase Polymerase Chain Reaction
- Ribonucleotide Reductases