Two genes differentially regulated in the cell cycle and by DNA-damaging agents encode alternative regulatory subunits of ribonucleotide reductase.

Abstract excerpt

Ribonucleotide reductase activity is essential for progression through the cell cycle, catalyzing the rate-limiting step for the production of deoxyribonucleotides needed for DNA synthesis. The enzymatic activity of the enzyme fluctuates in the cell cycle with an activity maximum in S phase. We have identified and characterized two Saccharomyces cerevisiae genes encoding the regulatory subunit of ribonucleotide...

Topics

• Amino Acid Sequence
• Blotting, Northern
• Cell Cycle
• DNA Damage
• DNA Replication
• DNA, Fungal
• Fungal Proteins
• Gene Expression Regulation, Fungal
• Genes, Fungal
• Molecular Sequence Data
• Mutation