Article
A mammalian cell line deficient in activity of the DNA repair enzyme 5-hydroxymethyluracil-DNA glycosylase is resistant to the toxic effects of the thymidine analog 5-hydroxymethyl-2'-deoxyuridine.
Molecular and cellular biology - 1 Dec 1992
Boorstein R J, Chiu L N, Teebor G W
Abstract excerpt
We isolated a mutant mammalian cell line lacking activity for the DNA repair enzyme 5-hydroxymethyluracil-DNA glycosylase (HmUra-DNA glycosylase). The mutant was isolated through its resistance to the thymidine analog 5-hydroxymethyl-2'-deoxyuridine (HmdUrd). The mutant incorporates HmdUrd into D...
Topics
- Animals
- Cell Division
- Cell Line
- Colony-Forming Units Assay
- Cricetinae
- DNA Glycosylases
- DNA Repair
- Drug Resistance
- N-Glycosyl Hydrolases
- Nucleosides
- Phenotype
- Thymidine