Article
Genetic engineering, isolation and characterization of a truncated Escherichia coli elongation factor Tu comprising domains 2 and 3.
Biochimica et biophysica acta - 23 Oct 1990
Pieper U, Ehbrecht H J, Fliess A, Schick B, Jurnak F, Pingoud A
Abstract excerpt
A deletion mutant of a plasmid born Escherichia coli tufA gene, which codes for a truncated elongation factor Tu comprising domains 2 and 3, has been constructed by genetic engineering. This gene was overexpressed in E. coli, and a polypeptide representing the truncated elongation factor Tu was isolated, purified to near homogeneity, crystallized and characterized physico-chemically as well as biochemically....
Topics
- Circular Dichroism
- Cloning, Molecular
- Crystallization
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Guanidine
- Guanidines
- Mutation