Article
Missense substitutions lethal to essential functions of EF-Tu.
Biochimie - 1 Dec 1991
Abdulkarim F, Tuohy T M, Buckingham R H, Hughes D
Abstract excerpt
We have used a simple selection and screening method to isolate function defective mutants of EF-Tu. From 28 mutants tested, 12 different missense substitutions, individually lethal to some essential function of EF-Tu, were identified by sequencing. In addition we found a new non-lethal missense mutation. The frequency of isolation of unique mutations suggests that this method can be used to easily isolate many...
Topics
- Base Sequence
- Binding Sites
- Drug Resistance, Microbial
- Genes, Bacterial
- Genes, Lethal
- Molecular Sequence Data
- Mutation
- Peptide Elongation Factor Tu
- Polymerase Chain Reaction
- Protein Biosynthesis
- Protein Conformation