Article
A new PCR method: one primer amplification of PCR-CTPP products.
Molecular biotechnology - 1 Oct 2012
Yin Guang, Mitsuda Yoko, Ezaki Takayuki, Hamajima Nobuyuki
Abstract excerpt
Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) is a convenient method for genotyping single nucleotide polymorphisms, saving time, and costs. It uses four primers for PCR; F1 and R1 for one allele, and F2 and R2 for the other allele, by which three different sizes of DNA are amplified; between F1 and R1, between F2 and R2, and between F1 and R2. To date, we have applied PCR-CTPP...
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