Article
Competitive amplification and unspecific amplification in polymerase chain reaction with confronting two-pair primers.
The Journal of molecular diagnostics : JMD - 1 May 2002
Hamajima Nobuyuki, Saito Toshiko, Matsuo Keitaro, Tajima Kazuo
Abstract excerpt
Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) is an inexpensive, time-saving genotyping method that is applicable for most single nucleotide polymorphisms. To date, we have applied PCR-CTPP successfully for the genotyping of more than 30 polymorphisms. This paper demonstrates the differences in DNA amplification among different annealing temperatures of PCR-CTPP with given melting...
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