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An Assay for Variable Fragment Length Allele-Specific Genotyping.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Kalendar Ruslan, Shevtsov Vladislav, Ismailova Aisulu

Abstract excerpt

Polymerase chain reaction (PCR) is a quick and easy technique used to detect nucleotide polymorphisms and sequence variations in various fields such as medicine, agriculture, and basic research. There exists a set of PCR variants, collectively called allele-specific PCR, which use competitive reactions in the presence of allele-specific primers to amplify only specific alleles. In this chapter, we present...

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PMID: 40580281
DOI: 10.1007/978-1-0716-4642-7_1

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