Article
Polymerase chain reaction with confronting two-pair primers for polymorphism genotyping.
Japanese journal of cancer research : Gann - 1 Sept 2000
Hamajima N, Saito T, Matsuo K, Kozaki K, Takahashi T, Tajima K
Abstract excerpt
A novel PCR method using confronting two-pair primers, named PCR-CTPP, is introduced to detect a single nucleotide polymorphism (base X or Y). One primer for the X allele is set to include X' at the 3' end (antisense), where X' is the antisense of X, with the counterpart sense primer upstream. For the Y allele, a sense primer including Y at the 3' end is set, with the antisense primer downstream. One common band...
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