Article
Triplex polymerase chain reactions with confronting two-pair primers (PCR-CTPP) for NQO1 C609T, GSTM1 and GSTT1 polymorphisms: a convenient genotyping method.
Asian Pacific journal of cancer prevention : APJCP - 1 Jan 2000
Kawase Haruya, Hamajima Nobuyuki, Tamakoshi Akiko, Wakai Kenji, Saito Toshiko, Tajima Kazuo
Abstract excerpt
The polymerase chain reaction with confronting two-pair primers (PCR-CTPP) is a time-saving and inexpensive genotyping method, which is applicable for most single nucleotide polymorphisms (SNPs). To date, we have established PCR-CTPP conditions for tens of SNPs, including duplex genotyping. This paper introduces triplex PCR-CTPP to simultaneously genotype three functional polymorphisms of carcinogen-detoxifying...
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