Article
[Establishment and preliminary application of polymerase chain reaction with confronting two-pair primers for the single nucleotide polymorphisms of metabolic enzymes].
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi - 1 Jan 2009
Fu Jing, Yang Yi-Rong, Ni Xiao-Jie, Pan Xiao-Dong, Zheng Jian-Jian, Zheng Shao-Ling, Lin Ren-Yu, Cai Ming, Chen Bi-Cheng
Abstract excerpt
OBJECTIVE: To develop a simple, accurate, rapid, economic, large-scale detection method for the detection of single nucleotide polymorphisms (SNPs) metabolic enzymes, using polymerase chain reaction with confronting two-pair primers (PCR-CTPP). METHODS: The primers of CYP1A1 (A4889G), EPHX1 (A416G) and NQO1 (C609T) were designed for PCR-CTPP, and the PCR conditions were optimized. The results of genotyping were...
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