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Article

An improved PCR-CTPP assay for the detection of ADH1B Arg48His polymorphism.

Journal of clinical laboratory analysis - 1 Feb 2018

Jiang Shupeng, Tong Yongqing, Zhao Rui, Xiong Ge, Qiao Bin, Li Yan

Abstract excerpt

BACKGROUND: ADH1B Arg48His polymorphism is associated with the development of alcohol-related diseases. In this study, we aimed to explore an improved polymerase chain reaction with confronting two-pair primers (PCR-CTPP) assay for the detection of ADH1B Arg48His polymorphism. METHODS: A mismatch was introduced at the 3' end of each of the two allele-specific to increase the specificity of the reaction. But...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 28657176
DOI: 10.1002/jcla.22268
PMCID: PMC6816835

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