Article
Confronting two-pair primer design for enzyme-free SNP genotyping based on a genetic algorithm.
BMC bioinformatics - 13 Oct 2010
Yang Cheng-Hong, Cheng Yu-Huei, Chuang Li-Yeh, Chang Hsueh-Wei
Abstract excerpt
BACKGROUND: Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) method produces allele-specific DNA bands of different lengths by adding four designed primers and it achieves the single nucleotide polymorphism (SNP) genotyping by electrophoresis without further steps. It is a t...
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