Article
Multiplex PCR with confronting two-pair primers for CYP1A1 Ile462Val, GSTM1, GSTT1, and NQO1 C609T.
Asian Pacific journal of cancer prevention : APJCP - 1 Jan 2000
Kawai Sayo, Nishio Kazuko, Nakamura Sakurako, Sekido Yoshitaka, Niwa Toshimitsu, Hamajima Nobuyuki
Abstract excerpt
Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) is an effective genotyping method for single nucleotide polymorphisms (SNPs) in aspects of reducing time and costs for analysis. So far we have established PCR-CTPP conditions for tens of SNPs, including a triplex genotyping (Kawase et al., 2003). In the present study we report a quadruplex PCR-CTPP to genotype simultaneously four functional...
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