Article
Molecular determinants of HIV-2 R5-X4 tropism in the V3 loop: development of a new genotypic tool.
The Journal of infectious diseases - 1 Jan 2012
Visseaux Benoit, Hurtado-Nedelec Margarita, Charpentier Charlotte, Collin Gilles, Storto Alexandre, Matheron Sophie, Larrouy Lucile, Damond Florence, Brun-Vézinet Françoise, Descamps Diane
Abstract excerpt
OBJECTIVE: The use of CCR5 inhibitors requires a tool to predict human immunodeficiency virus type 2 (HIV-2) tropism, as established in HIV-1. The aim of our study was to identify genotypic determinants of HIV-2 tropism located in the gp105 V3 loop. METHODS: HIV-2 tropism phenotypic assays were performed on 53 HIV-2 clinical isolates using GFP expressing human osteosarcoma T4 [GHOST(3)] cell lines expressing CD4...
Topics
- Base Sequence
- Cell Line, Tumor
- Genetic Association Studies
- Genotyping Techniques
- HIV-2
- Humans
- Molecular Sequence Data
- Mutation
- RNA, Viral
- Sequence Analysis, RNA