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Article

A sensitive phenotypic assay for the determination of human immunodeficiency virus type 1 tropism.

The Journal of antimicrobial chemotherapy - 1 Dec 2010

González Nuria, Pérez-Olmeda Mayte, Mateos Elena, Cascajero Almudena, Alvarez Amparo, Spijkers Sanne, García-Pérez Javier, Sánchez-Palomino Sonsoles, Ruiz-Mateos Ezequiel, Leal Manuel, Alcami José

Abstract excerpt

OBJECTIVES: To develop a sensitive phenotypic assay based on recombinant viruses (RVs) for characterizing HIV-1 tropism. METHODS: Viral tropism was assessed in 159 plasma samples. The env gene was amplified and ligated into pNL-lacZ/env-Ren, which carries a luciferase reporter gene. Resulting constructs were transfected into HEK293T cells to generate RVs. To assess co-receptor tropism, U87.CD4.CXCR4/CCR5 cells...

Topics

Cell Line, Tumor
Genes, Reporter
HEK293 Cells
HIV-1
Humans
Luciferases
Phenotype
Receptors, CCR5
Receptors, CXCR4
Recombination, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sensitivity and Specificity

Viral Tropism

env Gene Products, Human Immunodeficiency Virus

Source

Identifiers

PMID: 20947622
DOI: 10.1093/jac/dkq379

Genes

CCR5
CXCR4

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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