Article
Natural killer cells activated with interleukin 2 in vitro can be adoptively transferred and mediate hematopoietic histocompatibility-1 antigen-specific bone marrow rejection in vivo.
European journal of immunology - 1 Aug 1990
Murphy W J, Kumar V, Bennett M
Abstract excerpt
An adoptive transfer model was developed for examining natural killer (NK) cell-mediated bone marrow cell (BMC) graft rejection. Homogeneous populations of NK cells were obtained by culturing spleen cells from mice with severe combined immune deficiency in the presence of recombinant interleukin 2 for 7 days. These cells maintained a phenotypic (CD3-CD4-CD8-NK-2.1+ and asialo GM1+) and lytic pattern consistent...
Topics
- Animals
- Biomarkers
- Bone Marrow Transplantation
- Germ-Free Life
- Graft Rejection
- Immunization, Passive
- Interleukin-2
- Killer Cells, Natural
- Lymphocyte Activation
- Mice
- Phenotype