Article
Third position base changes in codons 5' and 3' adjacent UGA codons affect UGA suppression in vivo.
Biochimica et biophysica acta - 27 Aug 1990
Buckingham R H, Sörensen P, Pagel F T, Hijazi K A, Mims B H, Brechemier-Baey D, Murgola E J
Abstract excerpt
The base sequence around nonsense codons affects the efficiency of nonsense codon suppression. Published data, comparing different nonsense sites in a mRNA, implicate the two bases downstream of the nonsense codon as major determinants of suppression efficiency. However, the results we report here indicate that the nature of the contiguous upstream codon can also affect nonsense suppression, as can the third...
Topics
- Base Sequence
- Codon
- Escherichia coli
- Genes, Bacterial
- Genes, Suppressor
- Genotype
- Mutagenesis, Site-Directed
- RNA, Messenger