Article
Variety of nonsense suppressor phenotypes associated with mutational changes at conserved sites in Escherichia coli ribosomal RNA.
Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Jan 2000
Murgola E J, Pagel F T, Hijazi K A, Arkov A L, Xu W, Zhao S Q
Abstract excerpt
To screen for ribosomal RNA mutants defective in peptide chain termination, we have been looking for rRNA mutants that exhibit different patterns of suppression of nonsense mutations and that do not suppress missense mutations at the same positions in the same reporter gene. The rRNA mutations were induced by segment-directed randomly mutagenic PCR treatment of a cloned rrnB operon, followed by subcloning of the...
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