Article
Splice junction mutations in a yeast tRNA gene which alter the rate and precision of processing.
Biochimica et biophysica acta - 6 Apr 1990
Chambers J, Raymond G J, Kim D, Raymond K C, Nelson C, Clark S, Johnson J D
Abstract excerpt
We have introduced mutations into a tRNALeu3 gene which alter the intron boundaries and examined their effects on RNA splicing. Our results show that the 5'-proximal splice junction is not specified by the position of an adjacent base-paired stem present in all naturally occurring tRNA precursors. Also, efficient cleavage of 5'-splice junctions unique to these mutants, -CpU-, -UpA- and -UpG-, indicates the purine...
Topics
- Base Sequence
- Cell Nucleus
- DNA, Fungal
- Genes, Fungal
- Introns
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Nucleotide Mapping
- RNA Precursors
- RNA Splicing