Suppression of nonsense mutations induced by expression of an RNA complementary to a conserved segment of 23S rRNA.

Abstract excerpt

We identified a short RNA fragment, complementary to the Escherichia coli 23S rRNA segment comprising nucleotides 735 to 766 (in domain II), which when expressed in vivo results in the suppression of UGA nonsense mutations in two reporter genes. Neither UAA nor UAG mutations, examined at the same codon positions, were suppressed by the expression of this antisense rRNA fragment. Our results suggest that a stable...

Topics

• Base Sequence
• Chloramphenicol O-Acetyltransferase
• Codon, Terminator
• Conserved Sequence
• Escherichia coli
• Molecular Sequence Data
• Mutation
• Nucleic Acid Conformation
• RNA, Bacterial
• RNA, Ribosomal, 23S
• Suppression, Genetic