Synthesis of wild type and mutant human hemoglobins in Saccharomyces cerevisiae.

doi:10.1038/nbt0191-57

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Article

Synthesis of wild type and mutant human hemoglobins in Saccharomyces cerevisiae.

Bio/technology (Nature Publishing Company) - 1 Jan 1991

Wagenbach M, O'Rourke K, Vitez L, Wieczorek A, Hoffman S, Durfee S, Tedesco J, Stetler G

Abstract excerpt

We have expressed human alpha and beta-globin cDNA clones from separate, synthetic galactose-regulated hybrid promoters contained on a single plasmid in Saccharomyces cerevisiae. Co-expression of the alpha and beta-globin chains in S. cerevisiae results in the assembly of these proteins into solu...

Topics

Amino Acid Sequence
Galactose
Gene Expression Regulation
Globins
Hemoglobins
Humans
Molecular Sequence Data
Mutation
Oxygen
Recombinant Proteins
Saccharomyces cerevisiae

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