Article
Minimal deletion of amino acids from the carboxyl terminus of the B subunit of heat-labile enterotoxin causes defects in its assembly and release from the cytoplasmic membrane of Escherichia coli.
The Journal of biological chemistry - 5 Sept 1990
Sandkvist M, Hirst T R, Bagdasarian M
Abstract excerpt
Minimal alterations at the carboxyl terminus of the B subunit (EtxB) of heat-labile enterotoxin from Escherichia coli were found to have a marked effect on the assembly and release of this polypeptide into the periplasm. Nine mutant EtxB polypeptides were obtained by genetic manipulation of the 3...
Topics
- Amino Acid Sequence
- Bacterial Toxins
- Base Sequence
- Cell Membrane
- Chromosome Deletion
- Codon
- Enterotoxins
- Escherichia coli
- Escherichia coli Proteins
- Genes, Regulator
- Genetic Vectors
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Protein Biosynthesis
- Terminator Regions, Genetic