Article
Mutational analysis supports a role for multiple structural features in the C-terminal secretion signal of Escherichia coli haemolysin.
Molecular microbiology - 1 Oct 1991
Stanley P, Koronakis V, Hughes C
Abstract excerpt
We have carried out an extensive mutational analysis of the C-terminal signal which targets the export of the 1024-residue haemolysin protein (HlyA) of Escherichia coli across both bacterial membranes into the surrounding medium. Over 60 variants of the HlyA C-terminal 53-amino-acid sequence were created by oligonucleotide-directed mutagenesis and fused to the HlyA N-terminal 830 residues. Transport of the HlyA...
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