Expression of the B subunit of Escherichia coli heat-labile enterotoxin in a marine Vibrio and in a mutant that is pleiotropically defective in the secretion of extracellular proteins.

Abstract excerpt

A marine Vibrio (designated Vibrio sp. 60) that is related to Vibrio anguillarum was used as a host for a plasmid that encodes the non-toxic B subunit (EtxB) of Escherichia coli heat-labile enterotoxin. Expression of EtxB in Vibrio sp. 60 resulted in the efficient and selective secretion of the B subunit into the extracellular growth medium. This indicated that Vibrio sp. 60, which does not normally produce...

Topics

• Bacterial Proteins
• Bacterial Toxins
• Culture Media
• Enterotoxins
• Escherichia coli
• Escherichia coli Proteins
• Gene Expression
• Genetic Vectors
• Mutation
• Phenotype
• Plasmids