Article
Mapping intact protein isoforms in discovery mode using top-down proteomics.
Nature - 30 Oct 2011
Tran John C, Zamdborg Leonid, Ahlf Dorothy R, Lee Ji Eun, Catherman Adam D, Durbin Kenneth R, Tipton Jeremiah D, Vellaichamy Adaikkalam, Kellie John F, Li Mingxi, Wu Cong, Sweet Steve M M, Early Bryan P, Siuti Nertila, LeDuc Richard D, Compton Philip D, Thomas Paul M, Kelleher Neil L
Abstract excerpt
A full description of the human proteome relies on the challenging task of detecting mature and changing forms of protein molecules in the body. Large-scale proteome analysis has routinely involved digesting intact proteins followed by inferred protein identification using mass spectrometry. This 'bottom-up' process affords a high number of identifications (not always unique to a single gene). However,...
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