Article

Challenging the Astral <sup>™</sup> mass analyzer - up to 5300 proteins per single-cell at unseen quantitative accuracy to study cellular heterogeneity

2024-02-01

Abstract excerpt

<h4>ABSTRACT</h4> A detailed proteome map is crucial for understanding molecular pathways and protein functions. Despite significant advancements in sample preparation, instrumentation, and data analysis, single-cell proteomics is currently limited by proteomic depth and quantitative performance. We combine a zero dead-end volume chromatographic column running at high throughput with the Thermo Scientific™ Orbitr...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 23630187-9dfc-5265-9c63-aed70a5ca720
DOI: 10.1101/2024.02.01.578358

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Challenging the Astral <sup>™</sup> mass analyzer - up to 5300 proteins per single-cell at unseen quantitative accuracy to study cellular heterogeneityDOI 10.1101/2024.02.01.578358Read

Single-Cell Proteomics: Recent Advancement and ProspectsSemantic proximity Read

Ultra-high sensitivity mass spectrometry quantifies single-cell proteome changes upon perturbationSemantic proximity Read

An automated workflow for multiplexed single-cell proteomics sample preparation at unprecedented sensitivitySemantic proximity Read

Ultrasensitive single-cell proteomics workflow identifies >1000 protein groups per mammalian cellSemantic proximity Read

Mapping the human hematopoietic stem and progenitor cell hierarchy through integrated single-cell proteomics and transcriptomicsSemantic proximity Read

Comparative proteome signatures of trace samples by multiplexed Data-Independent AcquisitionSemantic proximity Read

Mass-spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiationSemantic proximity Read

Evaluating the capabilities of the Astral mass analyzer for single-cell proteomicsSemantic proximity Read

Mapping intact protein isoforms in discovery mode using top-down proteomics.Semantic proximity Read

Single Cell Proteomics Using a Trapped Ion Mobility Time-of-Flight Mass Spectrometer Provides Insight into the Post-translational Modification Landscape of Individual Human CellsSemantic proximity Read

Advancements in Single-Cell Proteomics and Mass Spectrometry-Based Techniques for Unmasking Cellular Diversity in Triple Negative Breast CancerSemantic proximity Read