Article
Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications.
Chemistry & biology - 28 Oct 2011
Adam Virgile, Moeyaert Benjamien, David Charlotte C, Mizuno Hideaki, Lelimousin Mickaël, Dedecker Peter, Ando Ryoko, Miyawaki Atsushi, Michiels Jan, Engelborghs Yves, Hofkens Johan
Abstract excerpt
Advanced fluorescence imaging, including subdiffraction microscopy, relies on fluorophores with controllable emission properties. Chief among these fluorophores are the photoactivatable fluorescent proteins capable of reversible on/off photoswitching or irreversible green-to-red photoconversion. IrisFP was recently reported as the first fluorescent protein combining these two types of phototransformations. The...
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