Article
Single amino acid replacement makes Aequorea victoria fluorescent proteins reversibly photoswitchable.
Journal of the American Chemical Society - 13 Jan 2010
Bizzarri Ranieri, Serresi Michela, Cardarelli Francesco, Abbruzzetti Stefania, Campanini Barbara, Viappiani Cristiano, Beltram Fabio
Abstract excerpt
Reversibly photoswitchable (i.e., photochromic) fluorescent proteins open the way to a number of advanced bioimaging techniques applicable to living-cell studies such as sequential photolabeling of distinct cellular regions, innovative FRET schemes, or nanoscopy. Owing to the relevance of fluorescent proteins from Aequorea victoria (AFPs) for cell biology, a photochromic "toolbox" constituted by several AFPs is...
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