Article
Isolation of a new class of 'flat' revertants from ras-transformed NIH3T3 cells using cis-4-hydroxy-L-proline.
Oncogene - 1 Aug 1990
Yanagihara K, Ciardiello F, Talbot N, McGeady M L, Cooper H, Benade L, Salomon D S, Bassin R H
Abstract excerpt
A new class of nontransformed revertant cells has been isolated from the ras-transformed cell line DT using cis-4-hydroxy-L-proline (CHP) as a selective agent. The new revertants, CHP 9CJ and CHP CB4, each contain two copies of the v-Ki-ras gene, elevated levels of phosphorylated p21ras protein, and rescuable transforming virus, indicating that the revertant phenotype observed in these cells does not result from...
Topics
- Animals
- Base Sequence
- Cell Line
- Cell Transformation, Neoplastic
- Genes, ras
- Humans
- Hydroxyproline
- Mice
- Ouabain
- Phenotype
- Phosphorylation
- Protein-Tyrosine Kinases