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[The cloning, expression, purification and immunological identification of wild-type and mutant hepatitis B virus X gene in pGEX-6P-2 system].

Bing du xue bao = Chinese journal of virology - 1 Sept 2011

Zhang Xiao-Hui, Wang Chang-Yuan, Tan Bing-Qin, Li Cheng, Du Lei, Wang Yong-Kang, Zhang Hong-Hua, Dong Ge-Feng

Abstract excerpt

To settle the foundation for the future research on the influence of wild and mutant (A1762T/ G1764A) HBV X gene on the progress of chronic HBV infection and hepatic tumorigenicity, wild and mutant (A1762T/G1764A) HBxAgs expression system was constructed. The wild and mutant (A1762T/ G1764A) HBV X genes were amplified with polymerase chain reaction (PCR) from HBV genome were inserted into pGEX-6P-2 and confirmed...

Topics

Antibodies, Neutralizing
Base Sequence
Cloning, Molecular
Escherichia coli
Gene Expression Regulation, Bacterial
Genetic Vectors
Hepatitis B, Chronic
Humans
Mutation
Recombinant Fusion Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Trans-Activators

Viral Regulatory and Accessory Proteins

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PMID: 21998953

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