Article
[Gene expression profiles and proteomics analysis of the cell transfected with the mutant type of COOH-terminal deleted of hepatitis B virus X].
Zhonghua yi xue za zhi - 21 Dec 2005
Liu Xiao-hong, Chen Ying, Wang Li, Zhao Hai-hua, Zhu Ming-hua
Abstract excerpt
OBJECTIVE: To investigate the mechanism of the different biological impacts of HBx3'-40, an engineered deletion mutant lacking the last 40 C-terminal amino acids. METHOD: Human hepatocellular cells of the line Huh7 were transfected with HBx3'-40 or wtHBx (wild type HBx) constructs. An oligo cDNA microarray containing 21074 human genes and Ests was utilized to examine the different gene expression between HBx3'-40...
Topics
- Carcinoma, Hepatocellular
- Cell Line, Tumor
- Electrophoresis, Gel, Two-Dimensional
- Gene Deletion
- Gene Expression Profiling
- Humans
- Mutant Proteins
- Mutation
- Oligonucleotide Array Sequence Analysis
- Proteomics