Article
[An analysis of the features of HBx protein distributed in liver cells and its expression in E. coli].
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology - 1 Jun 2006
Han Ju-qiang, Ding Li-hua, Yuan Bin, Wang Xiao-hui, Ning Kang, Li Jie-zhi, Lu Qiu-jun, Yang Xiao, Huang Cui-fen, Ye Qi-nong
Abstract excerpt
OBJECTIVE: To investigate the features of HBx protein distributed in liver cells and its expression in E. coli. METHODS: The expression vectors encoding the full length HBx and its mutants were constructed by the routine molecular cloning method. HBx protein expression was detected using Western blotting. The distribution feature of HBx protein in liver cells was examined using the fluorescence confocal...
Topics
- Carcinoma, Hepatocellular
- Cell Line
- Cloning, Molecular
- Escherichia coli
- Genetic Vectors
- Glutathione Transferase
- Hepatocytes
- Humans
- Liver
- Liver Neoplasms
