Article
Evaluating the sensitivity of Mycobacterium tuberculosis to biotin deprivation using regulated gene expression.
PLoS pathogens - 1 Sept 2011
Woong Park Sae, Klotzsche Marcus, Wilson Daniel J, Boshoff Helena I, Eoh Hyungjin, Manjunatha Ujjini, Blumenthal Antje, Rhee Kyu, Barry Clifton E, Aldrich Courtney C, Ehrt Sabine, Schnappinger Dirk
Abstract excerpt
In the search for new drug targets, we evaluated the biotin synthetic pathway of Mycobacterium tuberculosis (Mtb) and constructed an Mtb mutant lacking the biotin biosynthetic enzyme 7,8-diaminopelargonic acid synthase, BioA. In biotin-free synthetic media, ΔbioA did not produce wild-type levels of biotinylated proteins, and therefore did not grow and lost viability. ΔbioA was also unable to establish infection...
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