Article
Sequencing the gene for an imipenem-cefoxitin-hydrolyzing enzyme (CfiA) from Bacteroides fragilis TAL2480 reveals strong similarity between CfiA and Bacillus cereus beta-lactamase II.
Journal of bacteriology - 1 May 1990
Thompson J S, Malamy M H
Abstract excerpt
Using a newly constructed Bacteroides fragilis-Escherichia coli cloning shuttle vector, pJST61, we have cloned the cefoxitin (FOX)-imipenem (IMP) resistance determinant from B. fragilis TAL2480. FOX-IMP resistance in this strain results from the production of a periplasmic, Zn2(+)-containing beta...
Topics
- Amino Acid Sequence
- Bacillus cereus
- Bacterial Proteins
- Bacteroides
- Base Sequence
- Cephalosporinase
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Gene Library
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Plasmids
- Restriction Mapping
- Sequence Homology, Nucleic Acid