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Article

Induction of transformation and DNA synthesis after microinjection of raf proteins.

Molecular and cellular biology - 1 Jul 1990

Smith M R, Heidecker G, Rapp U R, Kung H F

Abstract excerpt

Full-length and N-terminal deletion mutants of human c-raf-1 cDNA were cloned into Escherichia coli expression plasmids. Bacterially expressed c-raf proteins were purified by anion-exchange, gel filtration, and affinity chromatography. Microinjection of mutant c-raf proteins into G0-arrested NIH 3T3 cells induced DNA synthesis and morphological transformation, whereas microinjection of full-length c-raf had no...

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Identifiers

PMID: 2192265
DOI: 10.1128/mcb.10.7.3828-3833.1990
PMCID: PMC360849

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Source receipt: pubmed, retrieved 7 Sept 2026.

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