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Article

Induction of transformation and DNA synthesis after microinjection of raf proteins.

Molecular and cellular biology - 1 Jul 1990

Smith M R, Heidecker G, Rapp U R, Kung H F

Abstract excerpt

Full-length and N-terminal deletion mutants of human c-raf-1 cDNA were cloned into Escherichia coli expression plasmids. Bacterially expressed c-raf proteins were purified by anion-exchange, gel filtration, and affinity chromatography. Microinjection of mutant c-raf proteins into G0-arrested NIH 3T3 cells induced DNA synthesis and morphological transformation, whereas microinjection of full-length c-raf had no...

Topics

Animals
Cell Transformation, Neoplastic
Cells, Cultured
Cloning, Molecular
DNA Replication
Escherichia coli
Humans
Mice
Microinjections
Mutation
Protein-Tyrosine Kinases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Proto-Oncogene Proteins

Proto-Oncogene Proteins c-raf

Recombinant Proteins

Restriction Mapping

Source

Open in PubMed Open full text

Identifiers

PMID: 2192265
DOI: 10.1128/mcb.10.7.3828-3833.1990
PMCID: PMC360849

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2192265-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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