Article
Interaction of adenovirus VA RNAl with the protein kinase DAI: nonequivalence of binding and function.
Cell - 1 Jun 1990
Mellits K H, Kostura M, Mathews M B
Abstract excerpt
Adenovirus VA RNAL maintains protein synthesis by preventing activation of the double-stranded RNA (dsRNA)-dependent protein kinase DAI. There appears to be a single binding site for dsRNA on DAI, and this site is blocked by VA RNAl. VA RNAl binds to purified DAI and can be cross-linked to the enzyme by UV irradiation. To determine the relationship between DAI binding and VA RNAl structure and function, we...
Topics
- Adenoviruses, Human
- Base Sequence
- Enzyme Activation
- Immunosorbent Techniques
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Protein Binding
- Protein Kinases
- RNA, Viral
- Structure-Activity Relationship