Article
The adenovirus VA RNA-derived miRNAs are not essential for lytic virus growth in tissue culture cells.
Nucleic acids research - 1 May 2013
Kamel Wael, Segerman Bo, Öberg Daniel, Punga Tanel, Akusjärvi Göran
Abstract excerpt
At late times during a lytic infection human adenovirus type 5 produces ∼10(8) copies per cell of virus-associated RNA I (VA RNAI). This short highly structured RNA polymerase III transcript has previously been shown to be essential for lytic virus growth. A fraction of VA RNAI is processed by Dicer into small RNAs, so-called mivaRNAIs, which are efficiently incorporated into the RNA-induced silencing complex....
Topics
- Adenoviruses, Human
- Base Sequence
- Genetic Vectors
- HEK293 Cells
- HeLa Cells
- Humans
- MicroRNAs
- Molecular Sequence Data
- Mutation
- RNA, Viral
- RNA-Induced Silencing Complex
- eIF-2 Kinase