Article
Chemical genetic analysis of protein kinase function in plants.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Böhmer Maik, Bölker Michael, Romeis Tina
Abstract excerpt
Identification of protein kinase targets and specific inhibition of individual kinase isoforms on the protein level in planta are important techniques to elucidate signal transduction pathways. The use of ATP-binding pocket mutants, the so-called gatekeeper mutants, that accommodate N(6)-enlarged nucleotides and kinase inhibitors has allowed a dramatic increase in kinase isoform selectivity. In this chapter, we...
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