Article
Screening of kinase substrates using kinase knockout mutants.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Umezawa Taishi
Abstract excerpt
Protein kinases are widely known to be major regulators of various signaling processes, particularly in eukaryotes, including plants. To understand their role in signal transduction pathways, it is necessary to determine which proteins are phosphorylated by these enzymes. Recent studies have applied a comparative phosphoproteomic approach to identify protein kinase substrates in plants. The results demonstrated...
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