Article
Design and use of analog-sensitive protein kinases.
Current protocols in molecular biology - 1 May 2004
Blethrow Justin, Zhang Chao, Shokat Kevan M, Weiss Eric L
Abstract excerpt
Many protein kinases can be engineered to accept analogs of ATP that are not efficiently used by wild-type kinases. These engineered kinases, which are referred to as "analog-sensitive" or "-as" alleles, are also often sensitive to protein kinase inhibitor variants that do not block the activity of nonmutant kinases. Selective in vitro use of radiolabeled ATP analogs by -as kinases can be exploited to identify...
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